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KMID : 0613820120220030360
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Journal of Life Science
2012 Volume.22 No. 3 p.360 ~ p.365
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Up-Regulation of NAG-1 and p21 Genes by Sulforaphane
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Jeong Byung-Geol
Kim Soon-Young
Lee Kon-Joo
Kim Jong-Sik
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Abstract
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We investigated the anti-proliferative activity of sulforaphane and expression changes of NAG-1 and p21 genes in response to sulforaphane treatment in human colorectal HCT116 cells. The results showed that sulforaphane decreased cell viabilities in a dose-dependent manner and induced expression of NAG-1 and p21 proteins in a dose-dependent and time-dependent manner. In addition, we found that NAG-1 expression by sulforaphane was not dependent on the presence of p53, whereas p21 expression was dependent on p53 presence. The results indicated that up-regulation of NAG-1 was not related with the activity of a dietary histone deacetylase inhibitor of sulforaphane. ATF3 induction was detected from 2 hr after sulforaphane treatment, indicating that ATF3 could be a transcription factor to up-regulate NAG-1 expression. The results of this study may help to increase our understanding of the molecular mechanism of anti-cancer activity mediated by sulforaphane in human colorectal cancer cells.
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KEYWORD
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Sulforaphane, anti-proliferative activity, NAG-1, p21, colorectal cancer cell
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